Useful in the study of cell & mitochondrial health, MTT is a pale yellow substrate that is cleaved by living cells to yield a dark blue formazan product. ... <看更多>
「mtt reagent」的推薦目錄:
mtt reagent 在 MTT Reagent 4890-25-01 - R&D Systems 的相關結果
The MTT Reagent (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide) is a component of TACS MTT Cell Proliferation Assay Kit. ... <看更多>
mtt reagent 在 MTT assay - Wikipedia 的相關結果
Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of ... ... <看更多>
mtt reagent 在 MTT assay protocol | Abcam 的相關結果
Ready to use reagents; Colorimetric; Validated on adherent cells and suspension cells; Quantitative Assay; Results in 3h 30 min. An ... ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay | ATCC 的相關結果
The MTT Reagent yields low background absorbance values in the absence of cells. For each cell type the linear relationship between cell number and signal ... ... <看更多>
mtt reagent 在 Is Your MTT Assay Really the Best Choice? - Promega ... 的相關結果
Although it is widely used, the MTT reagent exhibits cytotoxic effects, and adding the reagent to estimate cell viability may actually be damaging or even ... ... <看更多>
mtt reagent 在 MTT Assay - an overview | ScienceDirect Topics 的相關結果
The MTT assay is done to evaluate the cell viability of all cells in culture ... in 100 μL of media are incubated with 10 μL of MTT reagent (Cat # 30-1010K; ... ... <看更多>
mtt reagent 在 2,5-diphenyltetrazolium bromide) cell viability assay - Nature 的相關結果
Since MTT reagents are sensitive to light, MTT assays have to be done ... 10 μL of MTT reagent was added to the above mentioned medium and ... ... <看更多>
mtt reagent 在 MTT Reagent (4890-25-01) | Bio-Techne 的相關結果
The MTT Reagent (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide) is a component of TACS MTT Cell Proliferation Assay Kit. ... <看更多>
mtt reagent 在 MTT Reagent | CAS# 298-93-1 | 2809 | BioVision, Inc. 的相關結果
MTT is a tetrazolium salt used for determining cell viability. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT ... ... <看更多>
mtt reagent 在 The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation ... 的相關結果
These include the concentration of MTT reagent and the proportion that actually enters the cell, cellular metabolic activity (which is highly ... ... <看更多>
mtt reagent 在 MTT - Dojindo Molecular Technologies, Inc. 的相關結果
MTT is utilized for cell viability detection. Since MTT has a positive charge, it can pass through a cell membrane and is reduced by mitochondria to form a ... ... <看更多>
mtt reagent 在 MTT Reagent from R&D Systems | Biocompare.com 的相關結果
Compare MTT Reagent from R&D Systems on Biocompare.com. ... <看更多>
mtt reagent 在 Effect of growth media on the MTT colorimetric assay in bacteria 的相關結果
MTT assay. MTT reagent was prepared by dissolving 25 mg of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium ... ... <看更多>
mtt reagent 在 MTT - Cayman Chemical 的相關結果
Safety Data Sheet acc. to OSHA HCS. Printing date 11/03/2021. Revision date 11/03/2021. Trade name: MTT Reagent. (Contd. from page 1). ... <看更多>
mtt reagent 在 MTT Reagent - BioVision - Cambridge Bioscience 的相關結果
MTT Reagent - 5 x 1 g - 2809-5G from BioVision. ... is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. ... <看更多>
mtt reagent 在 MTT Supplier | CAS 298-93-1 | Tocris Bioscience 的相關結果
View and buy high purity MTT from Tocris Bioscience. Reagent used in the measurement of cell proliferation. ... <看更多>
mtt reagent 在 RD Systems MTT Reagent Quantity - Fisher Scientific 的相關結果
Fisher Scientific - R&D Systems MTT Reagent yields low background absorbance values in the absence of cells and is stable when stored at 4°C. Shop R&D ... ... <看更多>
mtt reagent 在 MTT - Biotium 的相關結果
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for ... ... <看更多>
mtt reagent 在 MTT Assay Protocol - 塞魯士有限公司 的相關結果
Reagent preparation and assay procedure for MTT assays to measure cell proliferation or cell cytoxicity. Reagent preparation. Prepare MTT solution: MTT is ... ... <看更多>
mtt reagent 在 MTT-Cell Based Proliferation/Toxicity Assay - Fivephoton ... 的相關結果
of MTT and its subsequent product. MTT is converted to. Formazan. Kit Contents. Catalog Number. Item. Quantity. Storage. MTT-R1. MTT Reagent (powder). ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay Kit (BN00550) 的相關結果
For background control, add 50 µl of MTT Reagent into a well containing media only (no cells). 8. Incubate the plate at 37°C for 3 hrs. 9. After incubation, add ... ... <看更多>
mtt reagent 在 MTT-Cell Based Proliferation/Toxicity Assay Kit - FineTest 的相關結果
The MTT Reagent yields low background absorbance values in the absence of cells. For each cell type the linear relationship between cell number and signal ... ... <看更多>
mtt reagent 在 Thiazolyl Blue (MTT) | Dye Reagent | MedChemExpress 的相關結果
Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan ... ... <看更多>
mtt reagent 在 細胞存活&細胞死亡專刊 - 伯森生技 的相關結果
Microplate Reader (Luminescent). Detection Method. Cell Counting Kit 8 (WST-8 / CCK8). WST-1 Assay Reagent. XTT Assay Kit. MTS Assay Kit. MTT Reagent. ... <看更多>
mtt reagent 在 EZcount MTT Cell Assay Kit - HiMedia 的相關結果
measured MTT reagent in the assay buffer, followed by its addition to the culture system. After dissolving the formazan crystals in the solubilization ... ... <看更多>
mtt reagent 在 MTT Reagent biochemical - MyBioSource 的相關結果
Buy MTT Reagent biochemical (MBS843090) product datasheet at MyBioSource, Biochemicals. ... <看更多>
mtt reagent 在 CytoSelect™ MTT Cell Proliferation Assay 的相關結果
Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular ... ... <看更多>
mtt reagent 在 MTT Assays | Springer Nature Experiments 的相關結果
Absorption spectra of MTT formazan reagent (25 μg/mL) in DMSO (A; Relationship between cell number and optical density in B16V melanoma cells (◯) and N592 ... <看更多>
mtt reagent 在 Analysis of Cell Viability by the MTT Assay - CSH Protocols 的相關結果
Reagents. Cultured mammalian cells that have been transfected. Most mammalian cell types reduce the tetrazolium salt sufficiently to perform MTT assays ... ... <看更多>
mtt reagent 在 Automated MTT assay for cell proliferation - INTEGRA ... 的相關結果
Increase your throughput by automating the MTT cell proliferation assay ... The use of INTEGRA's divided reagent reservoirs with SureFlo™ ... ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay Kit (Colorimetric) (NBP2-54883) 的相關結果
Store at -20C in the dark. Avoid freeze-thaw cycles. Kit Components. Components. MTT Reagent; MTT Solvent ... ... <看更多>
mtt reagent 在 MTT Reagent Clinisciences - 2809-1G 的相關結果
2809-1G | MTT Reagent. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex kits. Reagents and instruments for ... ... <看更多>
mtt reagent 在 mtt cell proliferation assay kit - Quimigen 的相關結果
Easy and Ready to use. Accurate: The absorbance signal produced correlates with the cell number. Convenient packaging. Economical. Size: MTT Reagent 10X - 10 x ... ... <看更多>
mtt reagent 在 Cell-Quant™ MTT Cell Proliferation Assay Kit | ABP Biosciences 的相關結果
MTT is an widely established reagent that is used to determine cell number in a variety of applications. Live cells reduce MTT to a strongly pigmented ... ... <看更多>
mtt reagent 在 MTT Cell Proliferation and Cytotoxicity Assay Kit Introduction 的相關結果
Add 10 μl MTT Reagent to each well, continue to culture for 4 hours. If drug react with MMT, you could centrifugal first then remove nutrient solution. ... <看更多>
mtt reagent 在 MTT Cell Proliferation Kit - OZ Biosciences 的相關結果
This MTT assay kit contains sufficient reagents to perform 1000 assays in 96-well plate format. Main features: Easy and Ready to use. Accurate: The absorbance ... ... <看更多>
mtt reagent 在 Cell culture | 細胞存活率試驗 的相關結果
MTT 分析法利用比色法測量細胞代謝活性,此方法為細胞活力、增殖和細胞毒性的指標,MTT與活細胞粒線體中的琥珀酸脫氫酶作用,產生氧化還原反應Formazan藍 ... ... <看更多>
mtt reagent 在 Alvetex Scaffold Protocol: MTT cell viability assay - REPROCELL 的相關結果
The MTT reagent alone results in very low background absorbance values in the absence of cells on Alvetex Scaffold. It is recommended that for each cell ... ... <看更多>
mtt reagent 在 MTT細胞增殖分析試劑盒 - 太鼎生物科技 的相關結果
MTT細胞增殖分析試劑盒(呈色法) | 貨號K299-1000 MTT Cell Proliferation Assay Kit (Colorimetric) 產品描述:活細胞數量的量化 ... Kit Components, • MTT Reagent ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay Kit - Westburg 的相關結果
Cells were serially diluted in a clear cell culture plate and incubated for 3 hrs with MTT Reagent at 37°C. After incubation, cells were treated with MTT ... ... <看更多>
mtt reagent 在 Improved Formazan Dissolution for Bacterial MTT Assay 的相關結果
The MTT reagent was prepared by dissolving 25 mg of MTT in 5 mL PBS. Ten microliters of MTT reagent was added to all wells, and plates were ... ... <看更多>
mtt reagent 在 MTT Reagent - Bertin Bioreagent 的相關結果
Check out MTT Reagent - €59.00 - Cayman - Bioreagent. ... <看更多>
mtt reagent 在 771 questions with answers in MTT ASSAY | Scientific method 的相關結果
MTT reagent exhibits cytotoxic effects and adding the reagent to estimate cell viability may actually be damaging or even killing cells during the incubation ... ... <看更多>
mtt reagent 在 Thiazolyl Blue Tetrazolium Bromide (MTT) | GoldBio 的相關結果
As the days count down to that perennial epic journey of one jolly... This vs. That – Your Guide to Choosing Between Similar Reagents. There are a lot of ... ... <看更多>
mtt reagent 在 Cell Meter™ Colorimetric MTT Cell Proliferation Kit - AAT ... 的相關結果
Intended use, Research Use Only (RUO) ; R-phrase, R20, R21, R22 ; UNSPSC, 12171501 ; Recommended plate, Clear bottom ; Component A: MTT Reagent A, 40 mL. ... <看更多>
mtt reagent 在 MTT Cell Proliferation and Cytotoxicity Assay Kit - Cloud-Clone 的相關結果
Specification, 1000 tests. Reagent A: MTT (powder). 50 mg. Reagent B: MTT solution. 10 ml. Reagent C: Formazan solution. 120 ml. Literature. ... <看更多>
mtt reagent 在 MTT Assay - iGEM 2013 的相關結果
Final concentration of MTT is 0.5 mg/ml. • Use 1xPBS to dissolve the MTT powder. • Filter it before use. Procedure. 1. After incubation of cells ... ... <看更多>
mtt reagent 在 Quantifying Cytotoxicity of Thiostrepton on ... - Agilent 的相關結果
mesothelioma cells using Invitrogen's Vybrant® MTT Cell Proliferation Assay Kit. Introduction ... Briefly, prior to addition of the MTT reagent microplates. ... <看更多>
mtt reagent 在 Optimization of some parametric values of MTT for ... - DergiPark 的相關結果
Optimum amount of MTT reagent and incubation time after MTT addition were determined as 10 µL and 4 h for 96 well plate, respectively. ... <看更多>
mtt reagent 在 Quantifying Cytotoxicity of Thiostrepton ... - BioTek Instruments 的相關結果
mesothelioma cells using Invitrogen's Vybrant® MTT Cell Proliferation Assay ... Briefly, prior to addition of the MTT reagent microplates. ... <看更多>
mtt reagent 在 MTT assay to evaluate the cytotoxic potential of a drug 的相關結果
This method uses colorimeter to determine the cell viability (Mosmann et al., 1983). The MTT reagent yields low background absorbance values in ... ... <看更多>
mtt reagent 在 MTT Assay - AbVideo™ - Support - Abnova 的相關結果
A demonstration on the procedure of using MTT assay to assess the viability and the proliferation of regular cells with absorbance detection at 595nm is ... ... <看更多>
mtt reagent 在 In vitro Cytotoxicity Assay with MTT Dye - Eurofins Deutschland 的相關結果
Shortly before the end of the incubation period, the cells are evaluated microscopically and the MTT reagent is added. The cells are incubated for ... ... <看更多>
mtt reagent 在 MTT cell proliferation assay - RCPN - Research Center for ... 的相關結果
MTT Reagent. 2-8°C. Detergent Reagent. 18-24°C. Procedure. Short 96 well assay: Each condition should be done in triplicate or more. ... <看更多>
mtt reagent 在 MTT Assay for Cytotoxicity - G-Biosciences 的相關結果
This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent ... ... <看更多>
mtt reagent 在 Cytotoxicity Assay (MTT) - Abbexa 的相關結果
Reagents · MTT Solution: Dissolve 500 mg MTT powder in 10 ml PBS. Stir with a magnetic stirrer for approx. 1 h in the dark. · Acidified Isopropanol –Add 50 ml 2 M ... ... <看更多>
mtt reagent 在 5 mg/mL MTT solution - Elabscience 的相關結果
Filter: Supplements and Reagents / Others. Manual MSDS ; Background. MTT(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) is widely used for ... ... <看更多>
mtt reagent 在 Mtt Reagent (Beijing Solarbio Science) | Bioz 的相關結果
Mtt Reagent, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... <看更多>
mtt reagent 在 碧云天-ST1537-1g-MTT (98%, Reagent grade)(MTT试剂级) 的相關結果
Name (Chinese), MTT. Name (English), MTT|Thiazolyl Blue Tetrazolium Bromide. Specifications, Reagent grade, 98%. Synonym (Chinese), 噻唑蓝, 3-(4,5-二甲基-2- ... ... <看更多>
mtt reagent 在 Assess cell viability and proliferation with colorimetric readouts 的相關結果
Although easy to run, the MTT assay does require two reagent addition and ... Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS ... ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay Kit 的相關結果
MTT Reagent (powder). 1 vial/25 mg. 1 vial/125 mg. 4°C. 10009322. Cell-Based Assay Buffer. Tablet. 1 tablet. 1 tablet. Room Temperature. 10009593. ... <看更多>
mtt reagent 在 Cellular viability using the MTT assay | RE-Place 的相關結果
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. ... <看更多>
mtt reagent 在 MTT Proliferation Assay Protocol.pdf - University of San Diego 的相關結果
沒有這個頁面的資訊。 ... <看更多>
mtt reagent 在 Toxicity of Layered Semiconductor Chalcogenides 的相關結果
(WST-8) and methyl-thiazolyldiphenyl-tetrazolium bromide (MTT) assays after a 24 h ... C and 5% CO2, whereas cells with the MTT reagent were. ... <看更多>
mtt reagent 在 MTT Solution [for Cell proliferation assay] (1mL×5) - TCI ... 的相關結果
This product consists of 5.0 mg / mL MTT dissolved in PBS. When 1/10th of the medium is added to cultured cells, ... Cell Biology Reagents. TCIMAIL. ... <看更多>
mtt reagent 在 Thiazolyl blue, 1 g | Vitality Staining - Carl Roth 的相關結果
MTT -reagent, used for detection of apoptosis. Stock solution: 5 mg/ml in PBS buffer solution, sterile filtrated (pH <5) Stability of stock solution: approx. ... <看更多>
mtt reagent 在 MTT 細胞增殖檢測試劑, MTT assay - 岑祥股份有限公司 的相關結果
岑祥股份有限公司提供的MTT 細胞增殖檢測試劑, MTT assay的詳細資訊。 ... CellCount MTT Assay Kit ... AlamarBlue Cell Viability Assay Reagent. ... <看更多>
mtt reagent 在 Mtt at Thomas Scientific 的相關結果
Mtt found in: MTT, MTT, MTT Cell Growth Assay Kit MTT, MTT Formazan, ... MTT Reagent A Useful in the study of cell & mitochondrial health, MTT is a pale… ... <看更多>
mtt reagent 在 MTT cell proliferation assay - SlideShare 的相關結果
Detergent is then added to the wells, solubilizing the crystals so the absorbance can. Reagent Storage MTT Reagent 2 - 8° C Detergent Reagent 18 - 24° C. ... <看更多>
mtt reagent 在 MTT assay - Labster Theory 的相關結果
MTT assay is a coloritmetric assay to measure cell survival. ... Add MTT reagent; Incubate for 2 hours at 37 °C until purple precipitates ... ... <看更多>
mtt reagent 在 MTT Cell Proliferation Assay | 蘋果健康咬一口 的相關結果
Detergent Reagent. 7. Record absorbance at 570 nm. MTT Cell Proliferation Assay. Instruction Guide. Component. Volume. Storage. MTT Reagent. 25 mL. 4°C. ... <看更多>
mtt reagent 在 Cell culture: MTT assay Flashcards | Quizlet 的相關結果
MTT assay protocol: Steps 1. Plate cells at 1,000 to 100,000 per well. 2. Incubate for 6 to 24 hours. 3. Add 10 μL MTT Reagent. ... <看更多>
mtt reagent 在 MTT Assay Protocol for Cell Viability and Proliferation 的相關結果
Isopropanol or DMSO) and the released, solubilised formazan reagent is measured by spectrophotometer. Since reduction of MTT can only occur ... ... <看更多>
mtt reagent 在 What is MTT cell proliferation assay? - Idcafe.net 的相關結果
The MTT Reagent yields low background absorbance values in the absence of cells. For each cell type the linear relation- ship between cell ... ... <看更多>
mtt reagent 在 Cell viability assays: why, what and how | CytoSMART 的相關結果
Figure 2 | MTT assay for cell viability is based on the principle that MTT is converted to formazan, leading to a shift in the emission spectrum ... ... <看更多>
mtt reagent 在 mtt アッセイ原理 - Gophr 的相關結果
The MTT Cell Proliferation Assay Reagent is a clear yellow ready-to-use solution, and it should be stored at -20 C protected from light. ... <看更多>
mtt reagent 在 What can be detected by MTT reaction? - Theburningofrome ... 的相關結果
Is MTT assay toxic? Toxicity. Although it is widely used, the MTT reagent exhibits cytotoxic effects, and adding the reagent to estimate cell ... ... <看更多>
mtt reagent 在 Biotechnology of Animal Tissues - 第 96 頁 - Google 圖書結果 的相關結果
Determination of the Viable Concentration of Cells by Dye Exclusion : Tetrazolium Assay Materials MTT reagent : 5 mg / ml of the tetrazolium dye , MTT ( 3- ... ... <看更多>
mtt reagent 在 Cellular Biotechnology - 第 144 頁 - Google 圖書結果 的相關結果
Determination of the Viable Concentration of Cells by Dye Exclusion : Tetrazolium Assay Materials MTT reagent : 5 mg / ml of the tetrazolium dye , MTT ( 3- ... ... <看更多>
mtt reagent 在 Diagnostic and Therapeutic Methods and Reviews 的相關結果
Observe plates under microscope during incubation to estimate optimum incubation time with MTT reagent. Presence of blue granules inside cells indicates ... ... <看更多>
mtt reagent 在 mtt assay步驟 - QFOF 的相關結果
Reagent Preparation. 細胞貼附後(6-8h) 或overnight 測DAY0,將medium 吸掉後加入MTT 混合medium(比例1:9 要避光) 總體積100ul,其他盤換一般medium 加MTT 的那盤避 ... ... <看更多>
mtt reagent 在 mtt assay 步驟 的相關結果
常用的tetrazolium salt中,MTT被還原後生成的formazan無法溶於培養基中,必須再用DMSO或isopropanol溶解,故多只用於. Reagent Preparation. MTT assay實驗流程CCK-8 ... ... <看更多>
mtt reagent 在 Animal Cell Culture and Technology - 第 105 頁 - Google 圖書結果 的相關結果
Protocol 5.7 Materials MTT reagent: 5 mg/ml of the tetrazolium dye (Sigma Chemical Co.), MTT (3(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in ... ... <看更多>
mtt reagent 在 New Technologies in Cancer Diagnostics and Therapeutics 的相關結果
MTT. Assay. All glioma cells were seeded at 1 × 104 cells in 100 μl of ... (MTT) reagent (Sigma-Aldrich, St. Louis, MO, the ratio of MTT reagent to the ... ... <看更多>
mtt reagent 在 mtt assay步驟 - Ryder 的相關結果
Reagent Preparation. 細胞代謝活性分析(metabolic activity assay). 原理是基於活細胞內的乳酸脫氫酶(lactate dehydrogenase)活性可將tetrazolium salt或Alamar ... ... <看更多>
mtt reagent 在 Comparative Diagnostic Pharmacology: Clinical and Research ... 的相關結果
Formulation: The vitality staining reagent MTT is prepared as a solution dispersed in serum-free and pH indicator-free growth media (e.g., 60 ml/300 ml as ... ... <看更多>
mtt reagent 在 Synthesis and in vitro assay of hydroxyxanthones as ... - Paperity 的相關結果
After 24 h, the MTT reagent was added, and the mixture was further incubated for an additional 3 h. Afterward, the sodium dodecyl sulfate (SDS) page was ... ... <看更多>
mtt reagent 在 What is the Difference Between MTT and MTS Assay 的相關結果
Does MTT kill cells? Although it is widely used, the MTT reagent exhibits cytotoxic effects, and adding the reagent to estimate cell viability may actually be ... ... <看更多>
mtt reagent 在 Global Residential Combined Heat and Power Market 2022 ... 的相關結果
... MTT Micro Turbine Technology BV; Qnergy; Sonic Development Inc ... Ltd., Energy Chemical, Bide Pharmatech Ltd., Adamas Reagent, Ltd. ... <看更多>
mtt reagent 在 MTT法_百度百科 的相關結果
MTT 法又称MTT比色法,是一种检测细胞存活和生长的方法。其检测原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲臜(Formazan)并沉积在 ... ... <看更多>
mtt reagent 在 Nanosecond-pulsed DBD plasma remedy on human ... 的相關結果
The outcomes of MTT assays carried out on the 24-h submit remedy of Jurkat and ... CellTiter 96 was used throughout this assay as a reagent. ... <看更多>
mtt reagent 在 MTT (3-(4,5-Dimethylthiazol-2-yl) - Thermo Fisher 的相關結果
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. ... <看更多>